BDNF anticorps (Biotin)

N° du produit ABIN465696

L’anticorps Lapin Polyclonal anti-BDNF a été validé pour WB, ELISA et Neut. Il convient pour détecter BDNF dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour BDNF anticorps (Biotin) (ABIN465696)

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BDNF est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-BDNF anticorps (Biotin)

Specificité: Human BDNF

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human BDNF.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hBDNF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. To detect hBDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human BDNF (LS-C104728) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur BDNF

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Autre désignation: BDNF

Sujet: Name/Gene ID: BDNF
Family: NGF Beta

Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin

ID gène: 627

UniProt: P23560

Pathways: Signalisation RTK, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity